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Gene expression with quantitative RT-PCR Following RNA extraction (RNeasy kit, Qiagen), reverse transcription (Transcriptor First Strand cDNA Synthesis Kit, Roche) and SYBR-green RT-PCR (Lightcycler 480, Roche), gene expression was normalized to GAPDH and analysed using the C t method

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Gunasekaran V, Yuvakkumar R, Ganesan R, Palapetta SC,Gurusamy H

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Oftentimes, too-high levels of oxidative stress and Glutathione depletion can lead to multiple diseases, including neurodegenerative disorders (Alzheimers, Parkinsons), pulmonary disease (asthma, COPD), immune disease (HIV, autoimmune disease), cardiovascular diseases (hypertensions), liver disease, cystic fibrosis, and aging